| Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca~(2+) |
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| 引用本文： | Ke Qiang DING 1,2,Zhen Bin JIA2,Qing Fei WANG2,Juan BAI2,Ru Ting TONG2,Xin Kui WANG1,Hui Bo SHAO3* 1Institute of Coal Chemistry,Chinese Academy of Science,Taiyuan 030001 2Department of Chemistry,Hebei Teachers University,Shijiazhuang 050016 3D. Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca~(2+)[J]. 中国化学快报, 2002, 13(4) |
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| 作者姓名： | Ke Qiang DING 1 2 Zhen Bin JIA2 Qing Fei WANG2 Juan BAI2 Ru Ting TONG2 Xin Kui WANG1 Hui Bo SHAO3* 1Institute of Coal Chemistry Chinese Academy of Science Taiyuan 030001 2Department of Chemistry Hebei Teachers University Shijiazhuang 050016 3D |
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| 作者单位： | Ke Qiang DING 1，2，Zhen Bin JIA2，Qing Fei WANG2，Juan BAI2，Ru Ting TONG2，Xin Kui WANG1，Hui Bo SHAO3* 1Institute of Coal Chemistry，Chinese Academy of Science，Taiyuan 030001 2Department of Chemistry，Hebei Teachers University，Shijiazhuang 050016 3D |
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| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
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| 摘 要： | Recently, the adsorption behavior of protein on solid substrate has attracted much attention for the reason that adsorption on a substrate is the first key step for other further electrochemical investigation1. Meanwhile the free adsorption could provide a relative well-ordered structure, with which some fundamental study could be carried out conveniently2. As we know, the electrochemical impedance spectroscopy (EIS) method has become one of the powerful techniques to detect the change of i…
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Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca2+ |
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| Ke Qiang DING,Zhen Bin JIA,Qing Fei WANG,Juan BAI,Ru Ting TONG,Xin Kui WANG,Hui Bo SHAO. Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca2+[J]. Chinese Chemical Letters, 2002, 13(4) |
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| Authors: | Ke Qiang DING Zhen Bin JIA Qing Fei WANG Juan BAI Ru Ting TONG Xin Kui WANG Hui Bo SHAO |
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| Abstract: | In this paper, the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method. The result reveals that the adsorption of the protein-calmodulin contains two steps, i.e., one short quick step followed by a slow one. The complexation of calmodulin with Ca2+ was also first probed using EIS technique, in which the complexation of CaM with Ca2+ could be reflected by the change of apparent membrane capacitance(Capp) clearly. In all above measurements, a redox couple Fe(CN)63-/ Fe(CN)64- was used as probing-pin to reflect all the changes occurring in the above process. Our work suggests that some biological processes of CaM could be studied using EIS method conveniently. |
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| Keywords: | Calmodulin (CaM) adsorption behavior Au substrate complexation electrochemical impedance spectroscopy. |
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