Determination of calcium in biological material The use of calcein as an indicator in the edta titration |
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| Authors: | Taft Y. Toribara and Larysa Koval |
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| Affiliation: | Department of Radiation Biology, University of Rochester, School of Medicine and Dentistry, Rochester, New York, U.S.A. |
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| Abstract: | The EDTA titration of calcium using the change in fluorescence of Calcein indicator under ultraviolet illumination at the end-point was very satisfactory for essentially pure calcium chloride solutions. In order to use this method for biological samples, it was found best to separate the calcium by an oxalate precipitation at a pH of 4.7, convert the calcium oxalate to calcium carbonate, and dissolve the calcium carbonate in hydrochloric acid. |
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