Optimization of the reversed-phase high-performance liquid chromatographic separation of the enantimers of a cationic chiral drug (tolperisone) on a heptakis(6-azido-6-deoxy) perphenylcarbamated β-cyclodextrin column |
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| Authors: | T Velmurugan C B Ching S C Ng Z W Bai T T Ong |
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| Institution: | (1) Department of Chemical and Environmental Engineering, National University of Singapore, 4 Engineering Drive 4, 117576, Singapore;(2) Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore;(3) Chemical and Process Engineering Centre, National University of Singapore, 4 Engineering Drive 4, 117576, Singapore |
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| Abstract: | Summary Heptakis(6-azido-6-deoxy) perphenylcarbamated β-cyclodextrin has been synthesized and chemically immobilized on silica gel
for use as a chiral stationary phase (PC-CSP) for analytical separation of the enantiomers of chiral drugs. Separation of
the enantiomers of tolperisone was studied by high-performance liquid chromatography under reversed-phase conditions. The
chromatographic conditions were optimized by varying mobile phase pH, composition, ionic strength, and velocity; 40:60 methanol-1%
triethylammonium acetate (TEAA) buffer, pH 5.5, was found to be the most suitable for this separation. |
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| Keywords: | Column liquid chromatography β -Cyclodextrin Enantimer separation Tolperisone Optimizatio |
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