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巯基修饰单链DNA在纳米金薄膜电极上自组装、杂交和取向的电化学交流阻抗谱
引用本文:魏旭,郝青丽,陆路德,汪信,杨绪杰. 巯基修饰单链DNA在纳米金薄膜电极上自组装、杂交和取向的电化学交流阻抗谱[J]. 应用化学, 2010, 27(8): 970-977. DOI: 10.3724/SP.J.1095.2010.90772
作者姓名:魏旭  郝青丽  陆路德  汪信  杨绪杰
作者单位:(南京理工大学材料化学实验室 南京 210094)
基金项目:江苏省科技支撑计划项目,南京理工大学卓越计划紫金之星项目 
摘    要:利用电化学交流阻抗技术对SH-ssDNA在纳米金薄膜电极表面的自组装、杂交和取向进行了系统表征。 探讨了SH-ssDNA的组装时间、浓度和链长对其自组装的影响,自组装15 h时电荷传递电阻Rct最大,表面覆盖率最高;研究了SH-ssDNA的浓度、链长以及与互补DNA的杂交方式对杂交反应的影响。 结果发现,随着单链浓度的增加,杂交后Rct的变化值逐渐降低,当SH-ssDNA为5 μmol/L时Rct值比杂交前增加了16%。 通过对阻抗谱数据模拟和分析,表明SH-ssDNA以垂直竖立取向在金电极表面形成均匀致密单分子层,杂交效率与SH-ssDNA的覆盖率密切相关。

关 键 词:交流阻抗谱  SH-ssDNA  自组装  杂交  取向  
收稿时间:2009-11-23
修稿时间:2009-12-29

Electrochemical Impedance Spectroscopy Study of SH-ssDNA Self-Assembling, Hybridization and Orientation on Thin-film Nana-gold Electrode
WEI Xu,HAO Qing-Li,LU Lu-De,WANG Xin,YANG Xu-Jie. Electrochemical Impedance Spectroscopy Study of SH-ssDNA Self-Assembling, Hybridization and Orientation on Thin-film Nana-gold Electrode[J]. Chinese Journal of Applied Chemistry, 2010, 27(8): 970-977. DOI: 10.3724/SP.J.1095.2010.90772
Authors:WEI Xu  HAO Qing-Li  LU Lu-De  WANG Xin  YANG Xu-Jie
Affiliation:(Materials Chemistry Laboratory,Nanjing University of Science & Technology,Nanjing 210094)
Abstract:The self-assembling, hybridization and orientation of SH-ssDNA on the surface of thin-film nano-gold electrodes were characterized systematically by means of the electrochemical impedance technique. The effects of assembling time, concentration and the length of SH-ssDNA on its self-assembling were investigated. The Rct and surface coverage reached the highest values when assembling time was 15 h. The influences of the concentration and length of SH-ssDNA and its hybridization method with complementary DNA on the hybridization reaction were also studied. The result shows the change of Rct decreases with the increase of the SH-ssDNA concentration. The Rct was increased by 16% when the concentration of SH-ssDNA was 5 μmol/L. The data simulation and analysis of impedance spectroscopy indicated that a unique self-assembled monolayer of SH-ssDNA was formed with an upright orientation on the nano-Au electrode surface. The result shows the hybridization efficiency is closely associated with the coverage of SH-ssDNA.
Keywords:SH-ssDNA
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