Determination of benzoylecgonine and cocaine in biologic fluids by automated gas chromatography |
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Authors: | P Jacob B A Elias-Baker R T Jones N L Benowitz |
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Institution: | Medical Service, San Francisco General Hospital Medical Center, CA 94143. |
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Abstract: | A method is described for extraction of the cocaine metabolite benzoylecgonine, conversion to the butyl ester derivative and gas chromatographic analysis using packed or capillary columns. Using a capillary column, cocaine and benzoylecgonine may be determined simultaneously. The extraction scheme has been designed to facilitate processing of large numbers of samples generated in pharmacokinetic studies. Structural analogues, m-toluylecgonine and m-toluylecgonine methyl ester, are used as internal standards. Concentrations as low as 10 ng/ml in 1-ml samples of plasma or urine are readily determined. Between-run coefficients of variation were 1.01% for cocaine and 4.18% for benzoylecgonine for concentrations of 75 and 350 ng/ml, respectively. |
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