Chemiluminescence derivatization of methylglyoxal using 2-aminonicotinic acid |
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Authors: | Ohba Y Sakata M Zaitsu K |
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Affiliation: | Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi, Fukuoka, Japan. |
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Abstract: | To develop a sensitive and selective chemiluminometric method for the determination of methylglyoxal, we used 2-aminonicotinic acid as the chemiluminescence derivatization reagent. 2-Aminonicotinic acid reacts with methylglyoxal in an acidic solution at 37 degrees C for 4 h and gave a chemiluminescence in N,N'-dimethylformamide containing sodium tert-butoxide. The detection limit (blank intensity plus three-times its standard deviation of methylglyoxal) for methylglyoxal is 233 fmol in the reaction mixture. |
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