Internal structure of magnetic endosomes |
| |
Authors: | C Rivière C Wilhelm F Cousin V Dupuis F Gazeau R Perzynski |
| |
Institution: | (1) Laboratoire des Liquides Ioniques et Interfaces Chargées, UMR CNRS-UPMC-ESPCI 7612, 140 rue de Lourmel, 75015 Paris, France;(2) Laboratoire Matière et Systèmes Complexes, UMR CNRS 7057, Université Paris 7, 140 rue de Lourmel, 75015 Paris, France;(3) Laboratoire Léon Brillouin, UMR CEA-CNRS 12, CEA- Saclay, 91191 Gif-sur-Yvette, France |
| |
Abstract: | The internal structure of biological vesicles filled with magnetic
nanoparticles is investigated using the following complementary analyses:
electronic transmission microscopy, dynamic probing by magneto-optical
birefringence and structural probing by Small Angle Neutron Scattering
(SANS). These magnetic vesicles are magnetic endosomes obtained via a
non-specific interaction between cells and anionic magnetic iron oxide
nanoparticles. Thanks to a magnetic purification process, they are probed at
two different stages of their formation within HeLa cells: (i) adsorption of
nanoparticles onto the cellular membrane and (ii) their subsequent
internalisation within endosomes. Differences in the microenvironment of the
magnetic nanoparticles at those two different stages are highlighted here.
The dynamics of magnetic nanoparticles adsorbed onto cellular membranes and
confined within endosomes is respectively 3 and 5 orders of magnitude slower
than for isolated magnetic nanoparticles in aqueous media. Interestingly,
SANS experiments show that magnetic endosomes have an internal structure
close to decorated vesicles, with magnetic nanoparticles locally decorating
the endosome membrane, inside their inner-sphere. These results, important
for future biomedical applications, suggest that multiple fusions of
decorated vesicles are the biological processes underlying the endocytosis
of that kind of nanometric materials. |
| |
Keywords: | 81 07 Pr Organic-inorganic hybrid nanostructures 78 20 Fm Birefringence 61 12 Ex Neutron scattering (including small-angle scattering) |
本文献已被 PubMed SpringerLink 等数据库收录! |
|