Off-line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time-of-flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis |
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Authors: | Laura Bindila Reinaldo Almeida Alistair Sterling Mark Allen Jasna Peter-Katalinić Alina Zamfir |
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Institution: | 1. Institute for Medical Physics and Biophysics, University of Münster, Robert Koch Strasse 31, D-48149 Münster, Germany;2. Advion BioSciences, Norwich, UK |
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Abstract: | Implementation and optimization of an off-line capillary electrophoresis (CE)/(−)nanoESIchip-quadrupole time-of-flight (QTOF) mass spectrometric (MS) and tandem MS system for compositional mapping and structural investigation of components in complex carbohydrate mixtures is described. The approach was developed for glycoscreening and applied to O-glycosylated peptides from urine of a patient suffering from α-N-acetylhexosaminidase deficiency, known as Schindler's disease. The fundamental issue of sensitivity, previously representing a serious drawback of the off-line CE/MS analysis, could be positively addressed by the off-line conjunction of CE with automated chip-based ESI-QTOF-MS to provide flexibility for CE/chip MS coupling and enhance structural elucidation of single components in heterogeneous mixtures. Copyright © 2004 John Wiley & Sons, Ltd. |
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Keywords: | capillary electrophoresis chip nanoelectrospray ionization quadrupole time-of-flight mass spectrometry automation glycoconjugates |
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