A micro procedure for the estimation of the protein content of mucus glycoproteins |
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| Authors: | Margaret A Carroll Alex Post Lee J Griggs John E Zarembo |
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| Institution: | Smith Kline and French Laboratories, 1500 Spring Garden Street, Philadelphia, Pennsylvania 19101, USA |
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| Abstract: | A sensitive automated procedure for the estimation of the protein content of glycoproteins has been developed using 0.5-mg samples (10–250 μg of protein). The method employs a modification of the conventional amino acid analysis using the unresolved neutral and acidic amino acid peak to estimate the total protein content. Hexosamines, sialic acid, and amide amino acids do not interfere. |
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