| 混浊介质多光子激发荧光显微成像的蒙特卡罗模拟 |
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| 引用本文： | 鲁强,曾绍群,骆清铭,阮玉.混浊介质多光子激发荧光显微成像的蒙特卡罗模拟[J].光学学报,2001,21(9):073-1078. |
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| 作者姓名： | 鲁强 曾绍群 骆清铭 阮玉 |
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| 作者单位： | 1. 华中理工大学生物医学光子学教育部重点实验室
2. 山西大学光电研究所量子光学教育部重点实验室 |
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| 基金项目： | 国家自然科学基金 (39870 2 0 5、5 9836 2 40、6 0 0 2 5 5 14)，高校博士点专项基金 (980 48733)资助课题 |
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| 摘 要： | 将蒙特卡罗方法与几何光线追踪技术相结合用于研究样品混浊特性对多光子激发蒌光显微成像的影响。推导了模拟多层样品显微成像的数学模型，给出了用程序框图表示的基本仿真系统骨架，对传统蒙特卡罗方法的改进有效地增强了对聚焦光束的模拟能力，初步的模拟结果说明了混浊介质散射特性对显微成像的显著影响。
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| 关 键 词： | 多光子激发 荧光显微术 蒙特卡罗方法 混浊介质 成像技术 |
Monte Carlo Simulation for MPE Fluorescence Microscopy in Turbid Media |
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| Lu Qiang,Zeng Shaoqun,Luo Qingming,Ruan Yu.Monte Carlo Simulation for MPE Fluorescence Microscopy in Turbid Media[J].Acta Optica Sinica,2001,21(9):073-1078. |
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| Authors: | Lu Qiang Zeng Shaoqun Luo Qingming Ruan Yu |
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| Abstract: | A numerical program, which combines Monte Carlo method with geometrical ray trace used to study the behaviour of multi photon excitation (MPE) fluorescence microscopic imaging through turbid media, is presented. The mathematical model used to microscopically image multi layered tissues is presented. The basic skeleton of the simulation program is demonstrated with flow chart. A skill is introduced to improve the traditional Monte Carlo method, which enhances the capability of simulation of the converging light. The prominent effect on microscopic imaging caused by scattering characteristics of turbid media is shown through preliminary simulation experiments. |
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| Keywords: | multi photon excitation fluorescence microscopy Monte Carlo method turbid media |
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