Diterpenoids from Aerial Parts of Clerodendranthus spicatus and Their Cytotoxic Activity

A chemical investigation of AcOEt‐soluble portion of the 95% EtOH extract of the aerial parts of Clerodendranthus spicatus led to the isolation and characterization of a new diterpenoid, named clerospicasin J ( 1 ). Structure elucidation of 1 was achieved by spectroscopic analyses, including 2D‐NMR. The diterpenoid 1 exhibited significant inhibitory activity on the proliferation of the SKOV3 cell lines. Moreover, the cytotoxicity of 1 against SKOV3 cells was related to apoptotis as confirmed by DAPI (=2‐(4‐amidinophenyl)‐1H‐indole‐6‐carboxamidine) nuclear staining and flow cytometry.