| 限制酶Bsp63 I的化学修饰与抑制动力学研究 |
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| 引用本文： | 董群,邹国林,曹新文,朱汝璠.限制酶Bsp63 I的化学修饰与抑制动力学研究[J].武汉大学学报(理学版),1996(2). |
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| 作者姓名： | 董群 邹国林 曹新文 朱汝璠 |
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| 作者单位： | 武汉大学生命科学学院 |
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| 摘 要： | 经亲和层析得到Ｂｓｐ６３Ｉ．用对氯汞苯甲酸、磷酸吡哆醛、２，３－丁二酮修饰该酶的巯基、赖氨酸、精氨酸残基，结果表明：这些基团均与该酶活性有关．动力学研究表明磷酸吡哆醛对酶的抑制是一种类反竟争性抑制．
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| 关 键 词： | 限制性核酸内切酶，化学修饰，抑制类型 |
CHEMICAL MODIFICATION AND INHIBITION KINETICS OF RESTRICTION ENDONUCLEASE BSp63I |
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| Dong Qun, Zou Guolin, Cao Xinwen, Zhu Rufan.CHEMICAL MODIFICATION AND INHIBITION KINETICS OF RESTRICTION ENDONUCLEASE BSp63I[J].JOurnal of Wuhan University:Natural Science Edition,1996(2). |
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| Authors: | Dong Qun Zou Guolin Cao Xinwen Zhu Rufan |
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| Abstract: | Restriction endonuclease Bsp63I has been isolated and purified by affinity chromatography. The role of specific amino acid residues in Bsp63I was determined by chemical modification. Sulfhydryl groups were modified with p-chloromercuribenzoic acid,lysineresidues with pyridoxal-5'-phosphate(PLP)and arginine residues with 2, 3-butanedione. Theresults show that these residues are related to activity of Bsp63I. Dynamic analysis showsthat the type of PLP inhibition is analogous anticompetitive inhibition. |
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| Keywords: | restriction endonuclease chemical modification inhibition type |
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