神经源性休克死亡家兔心肌组织差异蛋白分析

目的:采用比较蛋白质组学方法分析正常与神经源性休克死亡蛋白表达差异.方法:外力打击兔颈动脉窦建立神经源性休克死亡模型.提取死亡组与对照组在心室肌蛋白进行二维电泳和凝胶图像分析,对差异蛋白质用HPLC-Chip/Ms纳流液质联用技术进行序列分析,经spectrum Mill搜索UniProtKB/SWISS-PORT后签...

Analysis of differentially expressed proteins in the rabbits myocardimn which death of neurogenic shock

Objective： Analysis of proteins expressed differentially between neurogenic shock death group and normal control group by comparative proteomics analysis Methods To establish neurogenic shock death model by hitting on the carotid sinus in rabbits. To extract proteins from the left ventricle of death group and normal control group. After 2 - dimensional eleetrophoresis and PDQuest for gel image analysis, the MS/MS of the peptides from the diferential proteins digested by tripsin were analyzed by HPLC - Chip/MS and the original data were pre - processed by Spectrum Mill then searched in UniProtKB/SWKKS - PORT database. Results 5 proteins upr~dated significantly in established neurogenic shock death group were identified, including keratin 10, alpha- 2B adrenergic receptor , superoxide dismutsse, mitechondrial precursor, zinc fmger protein- 569, hsc70- interacting protein, and as well as 2 proteins downregulated significantly including： annexinXIIlb, tissue factor pathway inhibitor precursor. Conclusion The proteins expression have changed in neurogenic shock death group. The proteins which wereidentified are latent biomarkers and can offer possible diagnosticate datas of neurogenic shockdeath.