乳腺癌细胞exosomes对人脐静脉内皮作用的实验研究

目的:观察人乳腺癌细胞株MDA-MB-231细胞源exosomes对人脐静脉内皮细胞株(HUVECs)增殖、迁移能力及血管样结构形成的影响.方法:超速离心及密度梯度离心法提取exosomes;MTT法检测MDA-MB-231细胞源exosomes对HUVECs增殖的影响;Transwell小室法检测HUVECs与exo...

The experimental study of effect of exosomes derived from breast cancer cell line on human umbilical vein endothelial cells

Objective：To observe the effect of exosomes derived from MDA-MB-231 cell line on proliferation,immigration and capillary-like tube formation of Human Umbilical Vein Endothelial cells.Method：Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation,MTT assay was used to observe the effect of exosomes on proliferation of HUVECs;HUVECs were treated with exosomes for 24 h,the change of cell migration was detected by Transwell chamber method.The capillary-like tube formations by HUVECs were observed.Result：MTT result showed that the concentration range of exosomes significantly increased HUVECs proliferation in a concentration-and time-dependent manner（P0.01）;It may significantly enhance the migration of HUVECs after treated with 200 μ/ml exosomes for 24 h（P0.01）,and may significantly promoted the capability of capillary-like tube formation of HUVECs（P0.05）.Conclusion：Exosomes derived from MDA-MB-231 cell line significantly increased HUVECs proliferation in a concentration-and time-dependent manner and it may promote the migration and the capability of capillary-like tube formation of HUVECs.