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High-performance liquid chromatography of dihydroxyacetone as its bis-2,4-dinitrophenylhydrazone derivative
Authors:V. Ferioli  F. Vezzalini  C. Rustichelli  G. Gamberini
Affiliation:(1) Dipartimento di Scienze Farmaceutiche, Università di Modena, via Campi, 183, 41100 Modena, Italy
Abstract:Summary A rapid and simple HPLC method for the identification and determination of dihydroxyacetone, a tanning agent widely used in a variety of preparations, is presented. The method consists of the analysis of the formulated products after a derivatization reaction which enhances the response and the detection limit of the chromatographic analysis. The characterization and the elucidation of the spacial geometry of the synthesized derivative are described.
Keywords:Column liquid chromatography  Dihydroxyacetone  2,4-dinitrophenylhydrazine  Tanning creams
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