| 血卟啉及金属血卟啉与甲氧卞氨嘧啶相互作用的荧光光谱研究 |
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| 引用本文： | 张勇,雷亚春,刘滇生. 血卟啉及金属血卟啉与甲氧卞氨嘧啶相互作用的荧光光谱研究[J]. 光谱实验室, 2004, 21(3): 609-612 |
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| 作者姓名： | 张勇 雷亚春 刘滇生 |
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| 作者单位： | 山西大学化学化工学院,山西大学现代化学研究所,太原市,030006;山西大学化学化工学院,山西大学现代化学研究所,太原市,030006;山西大学化学化工学院,山西大学现代化学研究所,太原市,030006 |
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| 基金项目： | 山西省青年科技基金资助项目 (No.2 0 0 2 10 0 8) |
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| 摘 要： | 荧光光谱法研究了甲氧卞氨嘧啶与血卟啉及金属血卟啉的作用情况 ,考察了酸度对甲氧卞氨嘧啶荧光强度的影响。在 p H=11.0的 KH2 PO4- Na2 HPO4缓冲液中 ,甲氧卞氨嘧啶有较强的荧光峰 ,血卟啉及金属血卟啉对甲氧卞氨嘧啶有明显的识别作用 ,结合常数大于 10 5,结合比均为 1∶ 1。金属血卟啉与甲氧卞氨嘧啶的结合常数小于血卟啉与甲氧卞氨嘧啶的结合常数 ,并对识别机理进行了探讨。
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| 关 键 词： | 血卟啉及金属血卟啉 甲氧卞氨嘧啶 荧光光谱 结合常数 |
| 文章编号： | 1004-8138(2004)03-0609-04 |
| 修稿时间： | 2004-03-20 |
Interaction Between Metallohematoporphyrin and Hematoporphyrin with Trimethoprim by Fluorescence |
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| ZHANG Yong LEI Ya-Chun LIU Dian-Sheng. Interaction Between Metallohematoporphyrin and Hematoporphyrin with Trimethoprim by Fluorescence[J]. Chinese Journal of Spectroscopy Laboratory, 2004, 21(3): 609-612 |
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| Authors: | ZHANG Yong LEI Ya-Chun LIU Dian-Sheng |
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| Abstract: | The paper is about on the interactions between trimethoprim with hematoporphyrin and metallohematoporphyrin by fluorescence spectra.The pH conditions of interaction of trimethoprim with hematoporphyrin and metallohematoporphyrin were investigated in detail.The result shows that hematoporphyrin and metallohematoporphyrin exhibits significant recognition toward trimethoprim.And the binding constant of K HP was larger than that of K M-HP in KH 2PO 4-Na 2HPO 4 buffer solution.Mechanism of recognition was further discussed. |
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| Keywords: | Hematoporphyrin and Metallohematoporphyrin Trimethoprim Fluorescence Spectra Binding Constants. |
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