Dextran stabilized poly(methyl methacrylate) latex particles and their potential application for affinity purification of lectins |
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Authors: | C S Chern C K Lee Y J Tsai |
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Institution: | (1) Department of Chemical Engineering National Taiwan Institute of Technology 43 Keelung Road, Section 4 Taipei 106, Taiwan, Republic of China, TW |
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Abstract: | Stable poly(methyl methacrylate) latex particles (220–360 nm in diameter) using dextran as the protective colloid were prepared
and characterized in this study. Such an emulsion polymerization system follows Smith–Ewart Case III kinetics (i.e., the average
number of free radicals per particle is greater than 0.5) due to the relatively large latex particle size. The carbohydrate
content of these dextran modified emulsion polymers shows a maximum around 5% dextran based on total monomer weight. The latex
stability during polymerization is closely related to the carbohydrate content of these latex particles, and it is controlled
by the ratio of the thickness of the dextran adsorption layer to that of the electric double layer of the latex particles.
The critical flocculation concentration (CFC) of the latex products correlates well with the latex stability during polymerization.
The greater the total scrap produced during polymerization (i.e., the stronger the bridging flocculation), the lower is the
CFC of the latex products. The affinity precipitation of concanavalin A (a model lectin used in this study) by the dextran
modified PMMA latex particles is also illustrated in this study. The specifically adsorbed concanavalin A increases with the
carbohydrate content of the dextran modified latex particles.
Received: 9 December 1996 Accepted: 8 April 1997 |
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Keywords: | Emulsion polymerization poly(methyl methacrylate) dextranstabilizer concanavalin A affinity purification colloidal stability |
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