Simultaneous spectrophotometric determination of drugs in pharmaceutical preparations using multiple linear regression and partial least-squares regression, calibration and prediction methods
Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, University of La Laguna,E-3S071 La Laguna, Tenerife, Spain
Abstract:
Two new methods for the simultaneous determination of acetylsalicylic acid, acetaminophen and caffeine based on total absorbance measurements and their processing by multiple linear regession and partial least-squares regression are proposed. The concentration ranges used to construct the calibration matrix were 4.0-12.0, 2.0-10.0 and 0.9-6.0 μg ml?1 for acetylsalicylic acid, acetaminophen and caffeine respectively. The proposed methods were validated by using a set of synthetic sample mixtures and subsequently applied to the determination of the three active principles in three different pharmaceutical preparations.