Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase |
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Authors: | LingLing An LiBing Liu Shu Wang |
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Institution: | (1) Beijing National Laboratory for Molecular Sciences, and Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China |
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Abstract: | The cationic charged water-soluble polyfluorenes containing 2,1,3-benzothiadiazole (BT) units (P1–3) have been synthesized
and characterized. These polymers demonstrate intramolecular energy transfer from the fluorene units to the BT sites when
oppositely charged hyaluronan is added due to the formation of electrostatic complexes, followed by a shift in emission color
from blue to green or brown. Upon adding hyaluronidase, the hyaluronan is cleaved into fragments. The relatively weak electrostatic
interactions of hyaluronan fragments with polyfluorenes keep their main chains separated and energy transfer from the fluorene
units to the BT sites is inefficient, and the polyfluorenes recover their blue emissions. The complexes of conjugated polymers/hyaluronan
can be utilized as probes for sensitive and facile fluorescence assays for hyaluronidase. The new assay method interfaces
with the aggregation and light harvesting properties of conjugated polymers.
Supported by the “100 Talents” Program of Chinese Academy of Sciences, the National Natural Science Foundation of China (Grant
Nos. 20725308 & 20721061), and 973 Project (Grant Nos. 2006CB806200 & 2006CB932100) |
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Keywords: | conjugated polyelectrolytes energy transfer biosensors hyaluronidase |
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