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LC-MS-MS Determination of (S,R)-Penehyclidine in Rat Plasma |
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| Authors: | Li Changkun Li Jinglai Cui Mengxun Fu Fenghua Zhong Bohua Zhang Zhenqing |
| Institution: | 1.Department of Pharmacology, School of Pharmacy, Yantai University, 32 Qingquan Road, 264005, Shandong, People’s Republic of China ;2.Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People’s Republic of China ; |
| Abstract: | A sensitive and selective method for determination of (S,R)-penehyclidine in rat plasma by liquid chromatography-tandem mass spectrometry is described. The procedure employed the use of an internal standard (I.S.) and a simple protein precipitation step. The method developed was linear from 0.1 to 100 ng mL−1, with a sensitivity of 0.1 ng mL−1 as the lower limit of quantification. The intra- and inter-day assay accuracy (relative error) was within 8.27% and precision (RSD) was below 6.7%. It was successfully applied to pharmacokinetic studies of (S,R)-penehyclidine in rat plasma. |
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