Programmable aperture microscopy: A computational method for multi-modal phase contrast and light field imaging |
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| Institution: | 1. Smart Computational Imaging Laboratory (SCILab), Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China;2. Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China;1. Nanjing University of Science and Technology, School of Electronic and Optical Engineering, Xiaolingwei 200#, Nanjing 210094, China;2. Nanjing University of Science and Technology, Jiangsu Key Laboratory of Spectral Imaging & Intelligence Sense, Xiaolingwei 200#, Nanjing 210094, China;1. Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China;2. Smart Computational Imaging Laboratory (SCILab), Nanjing University of Science and Technology, Nanjing, Jiangsu Province 210094, China;3. Centre for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore;1. Brookhaven National Laboratory - NSLS II, 50 Rutherford Dr. Upton, NY 11973-5000, USA;2. School of Aeronautics and Astronautics, Sichuan University, Chengdu 610065, China;3. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;5. Jiangsu Key Laboratory of Spectral Imaging & Intelligence Sense, Nanjing University of Science and Technology, Nanjing 210094, China;1. School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China;2. Nanjing University of Science and Technology, Jiangsu Key Laboratory of Spectral Imaging & Intelligence Sense, Xiaolingwei 200#, Nanjing, 210094, China;1. Brookhaven National Laboratory - NSLS II, 50 Rutherford Dr., Upton, NY 11973-5000, USA;2. Jiangsu Key Laboratory of Spectral Imaging & Intelligence Sense, Nanjing University of Science and Technology, Nanjing 210094, China;3. College of Mechatronic Engineering and Automation, National University of Defense Technology, Changsha 410073, China;4. School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore |
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| Abstract: | We demonstrate a simple and cost-effective programmable aperture microscope to realize multi-modal computational imaging by integrating a programmable liquid crystal display (LCD) into a conventional wide-field microscope. The LCD selectively modulates the light distribution at the rear aperture of the microscope objective, allowing numerous imaging modalities, such as bright field, dark field, differential phase contrast, quantitative phase imaging, multi-perspective imaging, and full resolution light field imaging to be achieved and switched rapidly in the same setup, without requiring specialized hardwares and any moving parts. We experimentally demonstrate the success of our method by imaging unstained cheek cells, profiling microlens array, and changing perspective views of thick biological specimens. The post-exposure refocusing of a butterfly mouthpart and RFP-labeled dicot stem cross-section is also presented to demonstrate the full resolution light field imaging capability of our system for both translucent and fluorescent specimens. |
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| Keywords: | Microscopy Phase retrieval Multi-modal imaging Quantitative phase microscopy Light field microscopy |
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