Separation of progressive motile sperm from mouse semen using on-chip chemotaxis |
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Authors: | Ko Yong-Jun Maeng Joon-Ho Lee Byung-Chul Lee Seungwoo Hwang Seung Yong Ahn Yoomin |
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Institution: | Department of Mechanical Engineering, Graduate School, Hanyang University, Wangsimni-ro, Seongdong-gu, Seoul, Korea. |
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Abstract: | We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection. |
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