Measurement of dothiepin and its major metabolites in plasma by high-performance liquid chromatography. |
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| Authors: | P J Taylor B G Charles R Norris P Salm P J Ravenscroft |
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| Institution: | Department of Clinical Pharmacology, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia. |
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| Abstract: | This paper describes a reversed-phase high-performance liquid chromatographic method which will simultaneously measure dothiepin and its three major metabolites (northiaden, northiaden-S-oxide and dothiepin-S-oxide) in plasma using trimipramine as internal standard. Sample preparation involved a basic extraction using diethyl ether followed by an acid back-extraction. The method we report is linear over the range 50-1000 ng/ml (r = 0.999), for all analytes. Total imprecision is less than 11% (coefficient of variation) and accuracy is greater than 94% (n = 20). Recovery of analytes varied considerably from 51.7% for northiaden-S-oxide to 90.2% for dothiepin-S-oxide. |
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