| 多肽IS4溶液构象的进一步NMR研究 |
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| 引用本文： | 涂光忠,苗振伟,张日清,罗雪春,蒋云,赵南明. 多肽IS4溶液构象的进一步NMR研究[J]. 高等学校化学学报, 1999, 20(2): 264-267 |
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| 作者姓名： | 涂光忠 苗振伟 张日清 罗雪春 蒋云 赵南明 |
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| 作者单位： | 1. 生物膜与生物膜工程国家重点实验室清华大学生物科学与技术系, 北京 100084;2. 北京大学化学与分子工程学院, 北京 100871 |
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| 基金项目： | 国家自然科学基金攀登项目资助 |
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| 摘 要： | 利用二维核磁共振技术对多肽IS4中除Ser-1外的所有残基质子进行了指认.积分NOESY谱中的相关峰可得到距离约束.二面角的约束来自偶合常数3JNHα.由慢交换质子可得到氢键的约束.在进行距离几何程序计算时利用这些约束可得到一组构象,用能量最优化程序优化后的结果表明,多肽IS4在CF3CD2OH中的构象为α-螺旋.
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| 关 键 词： | 多肽IS4 2DNMR 多肽构象 结构计算 |
| 收稿时间： | 1998-01-19 |
Further NMR Investigation and Conformation Study of Polypeptide IS4 |
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| TU Guang-Zhong,MIAO Zhen-Wei,ZHANG Ri-Qing,LUO Xue-Chun,JIANG Yun,ZHAO Nan-Ming. Further NMR Investigation and Conformation Study of Polypeptide IS4[J]. Chemical Research In Chinese Universities, 1999, 20(2): 264-267 |
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| Authors: | TU Guang-Zhong MIAO Zhen-Wei ZHANG Ri-Qing LUO Xue-Chun JIANG Yun ZHAO Nan-Ming |
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| Affiliation: | 1. Biomembrane & Biomembrane Engineering National Laboratory, Department of Biological Science & Biotechnology, Tsinghua University, , Beijing, 100084;2. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 |
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| Abstract: | Two|dimensional {}+1H NMR technique was used to determine the solution structure of IS4. The cross|peaks volumes in NOESY spectra were used to generate proton|proton distance constraints. These data, combined with hydrogen bond constraints and angle constraints, were used to obtain a set of 10 structures by program DIANA. These structures were refined by unrestrained energy minimization using standard minimization program. The results indicated that the conformation of IS4 in CF-3CD-2OD is of |helix. |
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| Keywords: | 2DNMR |
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