Application of UV detection in HPLC in the total antioxidant potential assay |
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Authors: | Pawe? M Wantusiak Bronis?aw K G?ód |
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Institution: | 1. Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of Natural Sciences and Humanities, 08-110, Siedlce, Poland
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Abstract: | HPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products. ![></img> </span> </span></td>
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