| 苯磺酸顺阿曲库铵原料中的R-S异构体和S-S异构体的高效液相色谱法测定 |
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| 引用本文： | 高存强,韦艳丽,秦连港.苯磺酸顺阿曲库铵原料中的R-S异构体和S-S异构体的高效液相色谱法测定[J].化学研究,2010,21(1):80-82. |
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| 作者姓名： | 高存强 韦艳丽 秦连港 |
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| 作者单位： | 1. 江苏恒瑞医药股份有限公司,江苏,连云港,222002
2. 江苏豪森药业股份有限公司,江苏,连云港,222006 |
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| 基金项目： | 江苏恒瑞医药股份有限公司资助项目 |
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| 摘 要： | 采用手性色谱柱(Chiralcel OD-RH 150×4.0 mm,5μm),以0.05 mol/L的KPF6缓冲液(用磷酸调节pH值为3.0)-乙腈(体积比50∶50)为流动相,在流速1.0 mL.min-1、检测波长280 nm条件下分离和测定了苯磺酸顺阿曲库铵的R-S和S-S异构体.结果表明,利用高效液相色谱法可以实现苯磺酸顺阿曲库铵的R-S和S-S异构体的手性拆分及其快速、简便、准确测定.
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| 关 键 词： | 苯磺酸顺阿曲库铵 R-S异构体 S-S异构体 高效液相色谱 手性拆分 测定 |
Determination of R-S-isomer and S-S-isomer of Cisatracurium Besilate by High Performance Liquid Chromatography |
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| GAO Cun-qiang,WEI Yan-li,QIN Lian-gang.Determination of R-S-isomer and S-S-isomer of Cisatracurium Besilate by High Performance Liquid Chromatography[J].Chemical Research,2010,21(1):80-82. |
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| Authors: | GAO Cun-qiang WEI Yan-li QIN Lian-gang |
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| Institution: | 1. Jiangsu Hengrui Medicine Co. Ltd. , Lianyungang 222002, Jiangsu, China ;2. Jiangsu Haosen Pharmaceutical Co. Ltd., Lianyungang 222002, Jiangsu, China) |
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| Abstract: | A chiral column （Chiralcel OD-RH 150×4.0mm,5μm） was used to separate and determine the R-S isomer and S-S isomer of cisatracurium besilate at a flow rate of 1.0 mL·min^-1 and detection wavelength of 280 nm, in the presence of 0.05 mol/L KPF6 buffer solution （The pH value was adjusted to 3.0 with phosphoric acid solution） plus acetonitrile （volume ratio 50：50） as the mobile phase. Results indicate that the R-S isomer and S-S isomer of cisatracurium besilate can be efficiently separated by high performance liquid chromatography （HPLC）. And the HPLC method can also be used to determine the two types of isomers rapidly, simply, and accurately. |
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| Keywords: | cisatracurium besilate R-S-isomer SS-isomer high performance liquid chromatog- raphy chiral separation determination |
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