| 饲料中D-泛酸钙的超临界流体色谱测定 |
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| 引用本文： | 程劼,谢建春,苏晓鸥. 饲料中D-泛酸钙的超临界流体色谱测定[J]. 分析测试学报, 2010, 29(4). DOI: 10.3969/j.issn.1004-4957.2010.04.023 |
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| 作者姓名： | 程劼 谢建春 苏晓鸥 |
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| 作者单位： | 中国农业科学院农业质量标准与检测技术研究所,国家饲料质量监督检验中心,北京,100081;北京工商大学,化学与环境工程学院,北京,100048 |
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| 摘 要： | 采用超临界流体色谱(SFC)测定了饲料中D-泛酸钙的含量.在二氧化碳流动相中添加14.85%甲醇改性剂及0.15%三氟乙酸添加剂,流速2 mL/min,在Kromasil ODS柱上分离,检测波长为210 nm.结果表明D-泛酸钙能在3 min内完成分离,在1.0 ～25.0 mg/L范围内,样品的质量浓度与色谱峰面积呈良好的线性关系(r=0.999 5).方法的精密度良好,相对标准偏差在2.3%以内,回收率为89% ～98%.方法操作简单,结果准确,适合普及应用.
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| 关 键 词： | 饲料 D-泛酸钙 超临界流体色谱 |
Determination of D-calcium Pantothenate in Animal Feeds by Supercritical Fluid Chromatography |
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| CHENG Jie,XIE Jian-chun,SU Xiao-ou. Determination of D-calcium Pantothenate in Animal Feeds by Supercritical Fluid Chromatography[J]. Journal of Instrumental Analysis, 2010, 29(4). DOI: 10.3969/j.issn.1004-4957.2010.04.023 |
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| Authors: | CHENG Jie XIE Jian-chun SU Xiao-ou |
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| Affiliation: | CHENG Jie1,XIE Jian-chun2,SU Xiao-ou1(1.China National Feed Quality Control Center,Institute of Quality St,ards , Testing Technology for Agro-products,Chinese Academy of Agriculture Sciences,Beijing 100081,China,2.Chemical & Environmental Engineering School,Beijing Technology , Business University,Beijing 100048,China) |
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| Abstract: | A supercritical fluid chromatographic(SFC)method for the determination of D-calcium pantothenate in animal feeds was developed.The experimental conditions were investigated.The result indicated that the analysis D-calcium pantothenate could be performed in 3 min on the Kromasil ODS column using a mixture of CO2 and 14.85% methanol(contained 0.15% trifluoroacetic acid) as mobile phase at a rate of 2 mL/min.The UV detector was set at a wavelength of 210 nm.The calibration curve was linear in the range of 1.0-... |
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| Keywords: | animal feeds D-calcium pantothenate supercritical fluid chromatography |
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