Directed formation of micro- and nanoscale patterns of functional light-harvesting LH2 complexes |
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Authors: | Reynolds Nicholas P Janusz Stefan Escalante-Marun Maryana Timney John Ducker Robert E Olsen John D Otto Cees Subramaniam Vinod Leggett Graham J Hunter C Neil |
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Affiliation: | Department of Chemistry, University of Sheffield, UK. |
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Abstract: | The precision placement of the desired protein components on a suitable substrate is an essential prelude to any hybrid "biochip" device, but a second and equally important condition must also be met: the retention of full biological activity. Here we demonstrate the selective binding of an optically active membrane protein, the light-harvesting LH2 complex from Rhodobacter sphaeroides, to patterned self-assembled monolayers at the micron scale and the fabrication of nanometer-scale patterns of these molecules using near-field photolithographic methods. In contrast to plasma proteins, which are reversibly adsorbed on many surfaces, the LH2 complex is readily patterned simply by spatial control of surface polarity. Near-field photolithography has yielded rows of light-harvesting complexes only 98 nm wide. Retention of the native optical properties of patterned LH2 molecules was demonstrated using in situ fluorescence emission spectroscopy. |
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