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Characterization of recombinant antibodies for detection of TNT and its derivatives |
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| Authors: | Mladen Simonoviíc Svetlana Milosević-Zlatanović Nenad Milosavić Miroslav M. Vrvić Branislav Simonović |
| Affiliation: | (1) Institute of General and Physical Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia;(2) Faculty of Mathematics and Natural Sciences, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia;(3) Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, 11000 Belgrade, Serbia;(4) Faculty of Chemistry, University of Belgrade, Studentski Trg 12-16, 11000 Belgrade, Serbia |
| Abstract: | Diverse recombinant immunoreagents specific for TNT-derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP-protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT-analog TNP-Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies. |
| Keywords: | TNT assay antibody TNP-Tris recombinant detection |
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