A solid-phase fluorescent biosensor for the determination of phenolic compounds and peroxides in samples with complex matrices |
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Authors: | P V Rodionov I A Veselova T N Shekhovtsova |
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Institution: | 1. Chemical Department, The Lomonosov Moscow State University, Moscow, 119991, Russia
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Abstract: | A solid-phase fluorescent biosensor for the determination of phenolic compounds (simple substituted phenols and catecholamines) and peroxides has been developed. The biosensor has a simple construction and the analytical signal is measured directly in a biosensitive layer {peroxidase-chitosan} on the sensor surface. This approach allowed analyzing samples with complex matrices (including water-insoluble samples and nontransparent solutions) without their preliminary pretreatment. Two novel fluorescent indicator reactions for the determination of the above-mentioned analytes in wide concentration ranges (from nmol l?1 to mm l?1) which provided an analytical signal registration on a solid phase were proposed. The developed sensor was applied successfully for the analysis of urine, cosmetics, pharmaceuticals preparations, etc. |
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