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Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent |
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| Authors: | Sanhita Sinharay Gabriela Fernández‐Cuervo Jasmine P. Acfalle Mark D. Pagel |
| Affiliation: | 1. Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA;2. Department of Pharmaceutical Sciences, University of Arizona, Tucson, AZ, USA;3. Department of Medical Imaging, University of Arizona, Tucson, AZ, USA |
| Abstract: | A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity. |
| Keywords: | diagnostic agents enzyme kinetics kinetics NMR imaging sulfatases |