| 肝细胞癌、肝硬化患者血清中代谢物组研究 |
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| 引用本文： | 陆强,黄一红,丛辉,刘霞,高红昌,林东海,王惠民.肝细胞癌、肝硬化患者血清中代谢物组研究[J].分析化学,2009,37(2). |
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| 作者姓名： | 陆强 黄一红 丛辉 刘霞 高红昌 林东海 王惠民 |
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| 作者单位： | 1. 南通大学附属医院,南通大学检验系,江苏省实验诊断学重点学科,南通,226001
2. 中国科学院上海药物研究所,上海,201203 |
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| 基金项目： | 江苏省医学重点建设学科项目 |
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| 摘 要： | 采用核磁共振方法检测肝硬化、肝细胞癌(简称肝癌)患者和健康人血清中代谢物,研究3组血清代谢物组的差异.利用偏最小二乘法-判别式分析(partial least square-discriminant analysis, PLS-DA)对NMR谱数据进行模式识别分析,探讨利用基于1H NMR代谢组学技术诊断肝癌的可行性.结果表明,与健康人相比,肝硬化、肝癌患者血清中脂质(低密度脂蛋白和极低密度脂蛋白)、胆碱、乙酰乙酸等含量减少,谷氨酰胺、丙酮酸、苯丙氨酸、酪氨酸等含量增加.PLS-DA分析结果显示肝癌患者可与健康人、肝硬化患者鉴别开来,肝癌诊断灵敏度达921%,假阳性率为5.7%,优于血清甲胎蛋白(alpha-fetoprotein, AFP)检测.研究结果表明,基于1H NMR代谢组学技术结合PLS-DA的方法具有灵敏、准确、重复性好等优点,有助于肝癌早期诊断.
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| 关 键 词： | 肝细胞癌 肝硬化 血清 代谢物组 核磁共振 模式识别 |
Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis |
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| LU Qiang,HUANG Yi-Hong,CONG Hui,LIU Xia,GAO Hong-Chang,LIN Dong-Hai,WANG Hui-Min.Study on Metabonome in Human Serum with Hepatocellular Carcinoma or Liver Cirrhosis[J].Chinese Journal of Analytical Chemistry,2009,37(2). |
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| Authors: | LU Qiang HUANG Yi-Hong CONG Hui LIU Xia GAO Hong-Chang LIN Dong-Hai WANG Hui-Min |
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| Institution: | Affiliated Hospital of Nantong University;Medical Laboratory Department of Nantong University;Key Science of Jiangsu Laboratory Diagnostics;Nantong 226001;Shanghai Institute of Materia Medica;Chinese Academy of Sciences;Shanghai 201203 |
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| Abstract: | A comparison for the metabonome in serum samples from patients of LC(liver cirrhosis),HCC(hepatocellular carcinoma)and healthy volunteers were studied with the method of NMR(nuclear magnetic resonance spectroscopy).To explore the potential of applying 1H NMR-based metabonomics to diagnose HCC,PLS-DA(partial least square-discriminant analysis)was employed to perform the pattern recognition analysis of NMR spectra.In comparison with healthy volunteers,the result show that patients of LC and HCC were character... |
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| Keywords: | Hepatocellular carcinoma liver cirrhosis serum metabonome nuclear magnetic resonance spectroscopy pattern recognition |
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