稀土对大鼠尿液成分影响的核磁共振研究

目前，稀土生物效应的研究已进入了分子和细胞水平，但在活体动物内研究稀土的作用情况还不多见。稀土化合物随食物进入动物体内后，会对动物体内的器官、组织、细胞产生一定影响，将导致其体液容量、分布、电解质浓度等方面的变化。现代NMR技术是目前药理毒理学研究的有效手段之一，它具有简便、全面、快捷、非破坏性等优点，可用于对体液样品的检测和研究。本文采用现代核磁共振技术研究了稀土化合物硝酸镧灌胃给药后对Wistar大鼠尿液中代谢产物的影响，为阐明稀土化合物的毒理学机制和稀土的进一步开发利用提供理论依据。

Nuclear Magnetic Resonance Studies on the Effects of Rare Earth in Rats Urine

Male Wistar rats were administrated orally with La(NO 3) 3 at doses of 0.05, 0.2, 2.0, 10 and 20 mg/kg body weight. Urine was collected over a 24 h period after dosing. Resonances for a large number of low molecular weight metabolites were assigned in a high resolution 1H NMR spectra of rat urine. The variation of some low molecular weight metabolites in urine provided a sensitive measurement of Rare Earth induced renal and liver lesions, in which DMA, DMG, urea, Kg, TMAO, succinate, citrate and amino acids have been suggested as NMR markers for renal damage and ethanol, lactate, taurine as the markers for liver damage. The method could be applicable to study of the toxicological effects of other compounds and drugs.