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A sensitive immunosensor using colloidal gold as electrochemical label |
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| Authors: | Zhao-Peng Chen Zhao-Feng Peng Peng Zhang Xue-Fang Jin Jian-Hui Jiang Xiao-Bing Zhang Guo-Li Shen Ru-Qin Yu |
| Affiliation: | aState Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China bChemistry and Chemical Engineering College, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), Hunan Normal University, Changsha 410081, China cDepartment of Environmental Chemistry, Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai 264003, China |
| Abstract: | A sensitive immunosensor using colloidal gold as electrochemical label is described. In this method, the capture protein was first immobilized on a carbon paste electrode surface through passive adsorption to bind quantitatively with corresponding antigen and colloidal gold labeled antibody to perform a sandwich assay. To detect the amount of the colloidal gold captured on the electrode surface, the colloid was first oxidized electrochemically to produce AuCl4− ions which were adsorbed strongly on the electrode surface. Adsorptive voltammetry was then employed for the determination of the adsorbed AuCl4− ions. A linear relationship between reduction wave peak current and the antigen concentration (human IgG) from 10 to 500 ng/ml is obtained with a detection limit of 4.0 ng/ml. |
| Keywords: | Immunosensor Colloidal gold Electrochemical label Adsorptive voltammetry |
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