EFFECT OF nifA PRODUCT ON SUPPRESSION OF Nif~- PHENOTYPE OF gin MUTATION AND CONSTITUTIVE SYNTHESIS OF NITROGENASE IN Klebsiella pneumoniae 期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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EFFECT OF nifA PRODUCT ON SUPPRESSION OF Nif~- PHENOTYPE OF gin MUTATION AND CONSTITUTIVE SYNTHESIS OF NITROGENASE IN Klebsiella pneumoniae

作者姓名：	朱家璧俞冠翘江群益王丽雯沈善炯

作者单位：	Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai

摘要：	This paper describes the role of nifA product on the ammonia regulation of nitrogen fixation in K. pneumoniae. A plasmid carrying nifA gene under the promoter of tetracycline resistance gene was constructed. When this nifA carrying plasmid was introduced into a glnAG mutant, the Nif~-phenotype of this gln mutant was suppressed. Furthermore, when the plasmid was introduced into the wild type and glnAG mutant, derepression of nitrogenase synthesis in ammonia occurred in both strains and the products of nif genes can be detected by two-dimensional gel electrophoresis in the extracts of these ammonia-grown bacterial cells.The constitutive synthesis of nitrogenase in NH_4~ was also demonstrated in free living nitrogen-fixing bacteria, Enterobacter cloacae, when the bacteria received the plasmid carrying nifA gent from K. pneumoniae.
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