(1) School of Chemical Engineering, Purdue University, 47907 West Lafayette, IN;(2) Present address: The Upjohn Company, 49001 Kalamazoo, MI
Abstract:
Effectiveness factors were predicted from measurements of basic parameters made on single oligosaccharides, and the prediction
was compared to experimental effec tiveness factors for the reaction of each oligosaccharide in the immobilized enzyme catalyst.
Kinetic parameters were obtained for the hydrolysis of each oligosaccharide catalyzed by soluble glucoamylase, and were fit
with a subsite model equation capable of generalization to all sizes of oligosaccharide. Diffusion coefficients in free solution
were determined from movement out of a capillary tube. Spatial characteristics of the immobilized enzyme bed were obtained
from pulse response experiments, allowing the calculation of effective diffusivities. Experimental effectiveness factors plotted
against modulus were in reasonable agreement with the predictions.