Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe |
| |
| Authors: | Zhang Jiangyan Li Zhengping Wang Hui Wang Yucong Jia Hongxia Yan Jingli |
| |
| Institution: | Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, PR China. |
| |
| Abstract: | It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
