True detection limits in an experimental linearly heteroscedastic system.: Part 2

Despite much different processing of the experimental fluorescence detection data presented in Part 1, essentially the same estimates were obtained for the true theoretical Currie decision levels (Y_C and X_C) and true Currie detection limits (Y_D and X_D). The obtained experimental values, for 5% probability of false positives and 5% probability of false negatives, were Y_C = 56.0 mV, Y_D = 125. mV, X_C = 0.132 μg/mL and X_D = 0.293 μg/mL. For 5% probability of false positives and 1% probability of false negatives, the obtained detection limits were Y_D = 158. mV and X_D = 0.371 μg/mL. Furthermore, by using bootstrapping methodology on the experimental data for the standards and the analytical blank, it was possible to validate previously published experimental domain expressions for the decision levels (y_C and x_C) and detection limits (y_D and x_D). This was demonstrated by testing the generated decision levels and detection limits for their performance in regard to false positives and false negatives. In every case, the obtained numbers of false negatives and false positives were as specified a priori.