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Intracellular concentration map of magnesium in whole cells by combined use of X-ray fluorescence microscopy and atomic force microscopy
Authors:Stefano Lagomarsino  Stefano Iotti  Giovanna Farruggia  Alessia Cedola  Valentina Trapani  Michela Fratini  Inna Bukreeva  Andrea Notargiacomo  Lucia Mastrototaro  Chiara Marraccini  Andrea Sorrentino  Ian McNulty  Stefan Vogt  Daniel Legnini  Sangsoo Kim  Alessandra Gianoncelli  Jeanette A.M. Maier  Federica I. Wolf
Affiliation:1. IPCF-CNR –UOS Roma c/o Dip Fisica Universita’ “Sapienza”, P.le A. Moro, 2 Rome, Italy;2. Physics Department, Universita’ Sapienza, P.le A. Moro, 2 Rome, Italy;3. IFN-CNR – V. Cineto Romano, 42 00156 Rome, Italy;4. Dipartimento di Medicina Interna, dell''Invecchiamento e Malattie Nefrologiche Università di Bologna, Via Massarenti, 9 40138 Bologna, Italy;5. Dipartimento di Biochimica “G. Moruzzi” Università di Bologna, Via Irnerio, 48 40126 Bologna, Italy;6. Istituto di Patologia Generale - Università Cattolica del Sacro Cuore - Facoltà di Medicina "A. Gemelli" L.go F. Vito, 1 00168 Rome, Italy;7. Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA;8. SINCROTRONE TRIESTE, S.S.14 km 163.5 in Area Science Park, I-34149 Basovizza – Trieste, Italy;9. Dipartimento di Scienze Cliniche, Università di Milano, Via GB Grassi 74, 20157 Milan, Italy;10. Istituto Nazionale Biostrutture e Biosistemi – Rome, Italy;11. Shubnikov Institute of Crystallography, Leninskii prospekt 59, Moscow, 119333 Russia;12. Consorzio Nazionale Interuniversitario per le Scienze Fisiche della Materia - V. della Vasca Navale, 84, 00146 Rome, Italy
Abstract:We report a novel experimental approach to derive quantitative concentration map of light elements in whole cells by combining two complementary nano-probe methods: X-ray fluorescence microscopy (XRFM) and atomic force microscopy (AFM). The concentration is derived by normalizing point-by-point the elemental (here Mg) spatial distribution obtained by XRFM, by the thickness measured using AFM. The considerable difference between the elemental distribution and the concentration maps indicates that this procedure is essential to obtain reliable information on the role and function of elements in whole cells.
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