| 兔防御素NP1在大肠杆菌中的表达 |
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| 引用本文： | 徐文生,许杨.兔防御素NP1在大肠杆菌中的表达[J].南昌大学学报(理科版),2003,27(3):295-298. |
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| 作者姓名： | 徐文生 许杨 |
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| 作者单位： | 南昌大学中德联合研究院,食品科学教育部重点实验室,江西,南昌,330047 |
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| 摘 要： | 通过选用大肠杆菌偏好密码子对兔防御素基因进行改造，人工合成兔防御素基因并构建编码GST—NP1融合基因，从而在大肠杆菌中进行表达。研究表明：表达的蛋白质经过亲和层析洗脱纯化，SDS—PAGE电泳分析，在目的分子量处出现预期条带。灰度扫描分析表明，表达量最高可达细菌总蛋白的34．7％。经切割、复性后的防御素具有溶血活性。
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| 关 键 词： | 兔 防御素 大肠杆菌 基因表达 溶血活性 免疫系统 |
| 文章编号： | 1006-0464(2003)03-0295-04 |
| 修稿时间： | 2003年3月11日 |
THE EXPRESSION AND PURIFICATION OF FUSION PORTEIN GST-NP1 IN E. Coli |
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| XU Wen-sheng,XU Yangd Science,Nanchang University,Nanchang ,China.THE EXPRESSION AND PURIFICATION OF FUSION PORTEIN GST-NP1 IN E. Coli[J].Journal of Nanchang University(Natural Science),2003,27(3):295-298. |
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| Authors: | XU Wen-sheng XU Yangd Science Nanchang University Nanchang China |
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| Institution: | XU Wen-sheng,XU Yangd Science,Nanchang University,Nanchang 330047,China) |
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| Abstract: | The codons of rabbit defensin NP1 gene were modified to eliminate the E.Coli rear codons. The coding region of defensin NP1 was cloned into plasmid pGEX-4T-1, and the fusion protein was expressed in E.Coli JM109.Preliminary study showed that after affinity chromatography there are only one protein band on SDS-PAGE gel. The fusion protein was expressed up about34.7% of total bacterial proteins, and exhibited hemolysis activity after digestion by CNBr and renaturation. |
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| Keywords: | rabbit defensin NP1 E Coli gene expression hemolysis activity |
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