Mimesis of peroxidase by Mn-TMPyP in the catalytic fluorescence reaction of the homovanillic acid-hydrogen peroxide system |
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Authors: | Yun -Xiang Ci Fang Wang |
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Affiliation: | (1) Department of Chemistry, Peking University, 100871 Beijing, People's Republic of China |
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Abstract: | The fluorescence produced by the catalytic effect of the manganese (III)-tetrakis-(N-methylpyridinium)porphyrin complex (Mn-TMPyP) on the oxidation of homovanillic acid by hydrogen peroxide has been studied. The reaction product fluoresces at 424 nm (with excitation at 316 nm). Traces of hydrogen peroxide (1.3 × 10–7–2.4 × 10–6M) and glucose (1.5–5.0 g/ml) can be determined with good accuracy and reproducibility. The characteristics of the mimetic enzyme Mn-TMPyP have been compared with those of horseradish peroxidase. |
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Keywords: | fluorescence reaction peroxidase mimesis manganese porphyrin hydrogen peroxide glucose |
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