Hexylacrylate-based mixed-mode monolith,a stationary phase for the nano-HPLC separation of structurally related enkephalins |
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Authors: | Ayat Abbood Christine Herrenknecht Gaëlle Proczek Stéphanie Descroix Jordi Rodrigo Myriam Taverna Claire Smadja |
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Affiliation: | 1.Univ. Paris-Sud, UMR CNRS 8612, LPNSS, Faculté de Pharmacie,Chatenay-Malabry,France;2.Univ. Nantes, EA2160, Mer Molécules Santé (MMS) Faculté de Pharmacie,Nantes,France;3.ESPCI, Laboratoire Environnement et Chimie Analytique, UMR CNRS 7121,Paris Cedex 05,France;4.Univ. Paris-Sud-BioCIS, UMR CNRS 8076, Faculté de Pharmacie,Chatenay-Malabry,France |
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Abstract: | The potential of an in situ photopolymerized hexylacrylate-based monolithic stationary phase-bearing sulfonic acid groups was investigated by studying the chromatographic retention of small structurally related peptides (enkephalins) by nano-LC. Several retention mechanisms were highlighted. First, a reverse-phase chromatographic behavior toward neutral solutes due to hexylacrylate-moieties was demonstrated. Second, an evaluation of the influences of buffer pH suggested the involvement of a cation-exchange mechanism due to the presence of 2-acrylamido-2-methyl-1-propanesulfonic acid. This cation-exchange phenomenon was confirmed by the clear influence of Na+ concentration in the mobile phase on peptide retention. |
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