| 现场光化学荧光法测定酪氨酸 |
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| 引用本文： | 蔡维平,王细生,朱庆枝,王志红,许金钩. 现场光化学荧光法测定酪氨酸[J]. 分析化学, 2000, 28(12): 1535-1537 |
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| 作者姓名： | 蔡维平 王细生 朱庆枝 王志红 许金钩 |
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| 作者单位： | 厦门大学化学系,教育部分析科学重点实验室,厦门,361005 |
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| 基金项目： | 福建省自然科学基金资助项目。 |
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| 摘 要： | 在氨性缓冲溶液中，酪氨酸经２７５ｎｍ紫外光照射后，产生荧光二聚体，据此，建立了现场光化学荧光法测定酪氨酸的新方法。线性范围为２．０＊１０＾－７－２．０＊１０＾－５ｍｏｌ／Ｌ。检测限及精密度分别为３．２＊１０＾－９ｍｏｌ／Ｌ＾－１和１．７％。方法简便、快捷，对其它氨基酸有较好的选择性，可用于啤酒及氨基酸注射的测定。文章对光化学反应机理作了初步探讨。
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| 关 键 词： | 光化学 荧光法 酪氨酸 测定 啤酒 氨基酸注射液 |
In-situ Photochemical Fluorimetry of Tyrosine and Its-Determination |
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| Cai Weiping,Wang Xisheng,Zhu Qingzhi,Wang Zhihong,Xu Jingou. In-situ Photochemical Fluorimetry of Tyrosine and Its-Determination[J]. Chinese Journal of Analytical Chemistry, 2000, 28(12): 1535-1537 |
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| Authors: | Cai Weiping Wang Xisheng Zhu Qingzhi Wang Zhihong Xu Jingou |
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| Abstract: | Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4CI buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2. 0 x 10-7 - 2. 0 x 10-5 mol/L tyrosine. The detection limit is 3 .2 x 10-9 mol/L and relative standard deviation is 1 .7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed. |
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| Keywords: | Photochemical reaction fluorimetry tyrosine |
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