Isolation of the major O-glycosidically linked oligosaccharides obtained by alkaline borohydride degradation of human meconium glycoproteins |
| |
| Authors: | C Capon P Cache Y Leroy G Strecker J Montreuil B Fournet |
| |
| Institution: | Laboratoire de Chimie Biologique, Unité Associée au CNRS No. 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France. |
| |
| Abstract: | Neutral and acidic oligosaccharides derived from human meconium glycoproteins by alkaline borohydride degradation have been separated by high-performance liquid chromatography on a Micro-Pak anion-exchange column. In each class, oligosaccharides were purified by normal-phase (neutral and acidic oligosaccharides) and reversed-phase (neutral oligosaccharides) chromatography. Effective separations of neutral oligosaccharides and acidic oligosaccharides were achieved. |
| |
| Keywords: | |
|
|
