Speciation of organic and inorganic arsenic by HPLC-HG-ICP

This paper deals with the application of high performance liquid chromatography (HPLC), hydride generation (HG) and inductively coupled plasma atomic emission spectrometry (ICP) to determine four species of arsenic: As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA). The coupling conditions of HPLC-HG-ICP are given. Two anionic exchange columns (Nucleosil-5SB and Hamilton PRP X-100) are tested and the separation conditions optimized. Two acids (H₂SO₄ and HCl at different concentrations) are tested to obtain the hydrides. The proposed method is applied to determine four arsenic species in a synthetic matrix simulating a fish extract.