Biosensor for total cholesterol estimation using N-(2-aminoethyl)-3-aminopropyltrimethoxysilane self-assembled monolayer |
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Authors: | Sunil K Arya Monika Datta S P Singh Bansi D Malhotra |
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Institution: | (1) Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K.S. Krishnan Marg, New Delhi, 110012, India;(2) Department of Chemistry, University of Delhi, Delhi, 110007, India |
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Abstract: | Cholesterol oxidase (ChOx), cholesterol esterase (ChEt), and horseradish peroxidase (HRP) have been co-immobilized covalently
on a self-assembled monolayer (SAM) of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTS) deposited on an indium–tin–oxide (ITO) glass surface. These enzyme-modified
(ChOx-ChEt-HRP/AEAPTS/ITO) biosensing electrodes have been used to estimate cholesteryl oleate from 10 to 500 mg dL−1. The sensitivity, K
m value, and shelf-life of these ChEt-ChOx-HRP/AEAPTS/ITO biosensing electrodes have been found to be 124 nA mg−1 dL, 95.098 mg dL−1 (1.46 mmol L−1), and ten weeks, respectively. The ChEt-ChOx-HRP/AEAPTS/ITO bio-electrodes have been used to estimate total cholesterol in
serum samples.
Figure Covalent immobilization of enzymes onto AEAPTS/ITO surface using EDC/NHS chemistry
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Cholesterol oleate N-(2-Aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTS) Self-assembled monolayer (SAM) Cyclic voltammetry Biosensor |
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