Lignin peroxidase production by Streptomyces viridosporus T7A |
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Authors: | Leda M F Gottschalk Jacyara M B Macedo Elba P S Bon |
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Institution: | (1) Departamento de Bioquímica, IQ, CT, Universidade Federal do Rio de Janeiro, 21949-900, RJ, Brazil;(2) Departamento de Bioquímica, IBRAG, Universidade do Estado do Rio de Janeiro, 20551-013, RJ, Brazil |
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Abstract: | Lignin peroxidase (LiP) production cost should be reduced to justify its use in the control of environmental pollution. In
this work, we studied the enzyme production by Streptomyces viridosporus T7A using glucose or corn oil as a carbon source having 0.65% yeast extract as a nitrogen source. Enzyme activity, observed
using either 0.65% glucose or corn oil at 0.1, 0.5, and 1.0% concentration, was 300, 150, 300, and 200 U/L, respectively.
Although higher enzyme activity was obtained in both media containing 0.65% glucose and 0.5% corn oil, the use of corn oil
resulted in a better LiP stability. When combined carbon sources were used, higher values of enzyme activity (360, 350, and
225 U/L) were observed in media with 0.65% glucose and supplemented with 0.1, 0.5, and 1.0% corn oil, respectively. Although
the presence of both glucose and 0.5% corn oil is favorable for LiP production, satisfactory results in terms of enzyme production
and stability could be also observed using 0.5% corn oil as a sole carbon source, which may lead to reduced production costs
of the LiP enzyme. |
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Keywords: | Streptomyces viridosporus Lignin peroxidase enzyme production corn oil medium optimization |
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