Determination of Internucleotide JHN Couplings by the Modified 2D JNN-Correlated [N, H] TROSY |
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Authors: | Xianzhong Yan Xiangming Kong Youlin Xia Kong Hung Sze Guang Zhu |
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Institution: | Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, People's Republic of China |
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Abstract: | Recent developments in the direct observation of J couplings across hydrogen bonds in proteins and nucleic acids provide additional information for structure and function studies of these molecules by NMR spectroscopy. A JNN-correlated 15N, 1H] TROSY experiment proposed by Pervushin et al. (Proc. Natl. Acad. Sci. USA 95, 14147–14151, 1998) can be applied to measure hJHN in smaller nucleic acids in an E.COSY manner. However, it cannot be effectively applied to large nucleic acids, such as tRNATrp, since one of the peaks corresponding to a fast relaxing component will be too weak to be observed in the spectra of large molecules. In this Communication, we proposed a modified JNN-correlated 15N, 1H] TROSY experiment which enables direct measurement of hJHN in large nucleic acids. |
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Keywords: | hydrogen bond tRNA NMR TROSY JNN-correlated [15N 1H] TROSY |
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