a Department of Radiotherapy and Radiation Oncology, Ludwig-Maximilians-University, Munich, Germany
b LFL Laser - Research Laboratory, Department of Urology, Ludwig-Maximilians-University, Munich, Germany
Abstract:
The influence of light emitted from a diode laser centred at λ = 805 nm was investigated on murine skeletal myotubes (C2), normal urothelial cells (HCV29), human squamous carcinoma cells of the gingival muscosa (ZMK) and urothelial carcinoma cells (J82) in a computer-controlled irradiation chamber. Cells were treated with varying fluences between 0 and 20 J cm?2. The response was tested by analysis of the mitotic index using single cell counting after Orcein staining and proliferation index based on BrdU incorporation during DNA synthesis. While the mitotic index of C2, HCV29 and J82 cells increased at a fluence of 4 J cm?2, irradiation with fluences of 20 J cm?2 resulted in a slight decrease. ZMK tumor cells showed a decrease of the mitotic index with both fluences. No significant differences could be determined when using irradiances between 10 mW cm?2 and 150 mW cm?2. The BrdU test after irradiation showed no significant effects compared to the controls in each cell line.