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Sandwich immunoassay for alpha-fetoprotein in human sera using gold nanoparticle and magnetic bead labels along with resonance Rayleigh scattering readout |
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| Authors: | Yao Lu Xiangyi Huang Jicun Ren |
| Institution: | 1. College of Chemistry & Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, People’s Republic of China |
| Abstract: | We describe a sensitive sandwich immunoassay for alpha-fetoprotein (AFP). It is making use of gold nanoparticles (GNPs) and magnetic beads (MBs) as labels, and of resonance Rayleigh scattering for detection. Two antibodies were labeled with GNPs and MBs, respectively, and MB-antigen-GNP complexes were formed in the presence of antigens. The MB labels also serve as solid phase carriers that can be used to magnetically separate the immuno complex. The GNP labels are used as optical probes, and Rayleigh scattering was used to determine the concentration of free GNPs-antibody after separation of the MB-antigen-GNP complexes. The concentration of AFP is related to the intensity of light scattered by free GNPs in the 13.6 pM to 436 pM concentration range, and the limit of detection is 13.6 pM. The method was applied to the determination of AFP in sera of cancer patients, and the results agree well with those obtained by conventional ELISA. A sensitive sandwich immunoassay for alpha-fetoprotein (AFP) was reported in this paper. It was based on high resonance Rayleigh scattering light of gold nanoparticles (GNPs) and rapid separation of magnetic beads (MBs). Rayleigh scattering intensity of free GNPs was reduced strongly after immunoassay. Under optimized conditions, we got good relationship between resonance Rayleigh scattering (RRS) of free GNPs and the AFP concentration to determine AFP concentration efficiently. |
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